Optimization of Total Flavonoid Extraction From the Helicteres hirsuta Lour. Roots by Bath Ultrasound Assisted method and cytotoxic activities of these Flavonoids

This study was carried out to optimize the various approaches analyze effects of variables on total flavonoid content extraction from roots Helicteres hirsuta L. The existence compounds in methanol fraction accessed by using LC-MS/MS analysis. results identified ideal parameters such as times (30 minutes); solvent concentration (50%); ultrasonic frequency (12 Hz); and material/solvent ratio [1:30 (w/v)] for extracting highest flavonoids H. Hirsuta. study's suggested that value 3.52684 (mg Catechin/g extract). verified experiment obtained an actual 5.205 Further, presence 20 a nature (66.667%) appearing purified fractional extract. These can inhibit DPPH free radicals at 50%, with IC50 536.760 g/mL, they also have inhibitory activity growth cancer cell lines values ranging 115.81 219.17g/mL. human leukemia line (HL-60) exhibits most significant cytotoxic response extract root g/mL.